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Synthetic peptide (Human)
Our Abpromise guarantee covers the use of ab53041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC (PFA fixed)||1/3000.|
IF image of ab53041 staining cryopreserved rat cortical neurons grown for 20 to 29 DIV.
Right panel showing a non treated culture (Control).
Left panel showing cultures treated with Triodothyronine (T3), 60ng/mL, starting at 10 DIV.
Cells were fixed with 4% paraformaldehyde containing 0.2% picric acid in 0.1M phosphate buffer, pH 6.9 for 20 minutes.
The cells were then incubated at 4°C over night with ab53041 (1:200) diluted in PBS containing 0.3% Triton-X 100 (PBSTx).
The secondary antibody used was ab150068 (1:200).
ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.
ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.
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