製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human Olig2 peptide (ab111666). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration. PubMed: 20025521
IHC-Fr 1/200. (see Abreview and notes)
IHC (PFA fixed) 1/1000.

ターゲット情報

  • 機能Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
  • 組織特異性Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
  • 関連疾患Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • ドメインThe bHLH is essential for interaction with NKX2-2.
  • 細胞内局在Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic domain helix loop helix protein class B 1 antibody
    • Basic helix loop helix protein class B 1 antibody
    • BHLHB antibody
    • bHLHB1 antibody
    • bHLHe19 antibody
    • Class B basic helix loop helix protein 1 antibody
    • Class B basic helix-loop-helix protein 1 antibody
    • class E basic helix loop helix protein 19 antibody
    • Class E basic helix-loop-helix protein 19 antibody
    • Human protein kinase C binding protein RACK17 antibody
    • Olig2 antibody
    • OLIG2_HUMAN antibody
    • Oligo2 antibody
    • Oligodendrocyte lineage transcription factor 2 antibody
    • Oligodendrocyte specific bHLH transcription factor 2 antibody
    • Oligodendrocyte transcription factor 2 antibody
    • OTTHUMP00000067569 antibody
    • OTTHUMP00000067570 antibody
    • PRKCBP2 antibody
    • Protein kinase C binding protein 2 antibody
    • Protein kinase C binding protein RACK17 antibody
    • Protein kinase C-binding protein 2 antibody
    • Protein kinase C-binding protein RACK17 antibody
    • RACK17 antibody
    see all

Anti-Olig2 antibody 画像

  • Anti-Olig2 antibody (ab42453) at 0.5 µg/ml (in 5% skim milk/PBS buffer) + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,38 kDa. We are unsure as to the identity of these extra bands.
  • ab42453 at a 1/100 dilution staining Olig2 in human brain tissue (pontine base) by Immunohistochemistry (frozen sections) incubated for 1 hour at 20°C. Acetone fixed. Blocked with 10% milk for 30 minutes at 20°C. Secondary used at 1/200 pig polyclonal conjugated to biotin.

    Note nuclear and cytoplasmic staining of oligodendrocytes in the white matter and neurons in the grey matter.

    See Abreview

Anti-Olig2 antibody (ab42453) 使用論文

This product has been referenced in:
  • Kiely AP  et al. a-Synucleinopathy associated with G51D SNCA mutation: a link between Parkinson's disease and multiple system atrophy? Acta Neuropathol 125:753-69 (2013). ICC/IF ; Human . Read more (PubMed: 23404372) »
  • Wang K  et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF ; Rhesus monkey . Read more (PubMed: 20025521) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA+NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step None
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization Yes - triton 0.2%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 19 2013

Gracias por contactarnos de nuevo.

Lamento mucho que sigáis teniendo problemas para detectar específicamente oligodendrocitos en vuestras muestras.

Revisando de nuevo el protocolo, y las imágenes enviadas, me gu...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E10.5 mouse embryo sections)
Specification E10.5 mouse embryo sections
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 08 2012

Thank you very much for contacting us.

If you could specify which image you are referring to, I can try to help you further. However, regarding the references, I am sorry to let you know that we do not have unrestricted access to all journals...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain - pontine base)
Specification Brain - pontine base
Fixative Acetone
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
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投稿 Dec 15 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain - frontal cortex)
Specification Brain - frontal cortex
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH6), EDTA (pH8) and dionized water
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 23 2009

Thank you for your patience in this matter. I have received a reply from the originator of ab42453 (Olig2 antibody). The exact immunogenic peptide sequence is proprietary information. However, we can state that it is within the following 50 amino...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"