All lanes : Anti-OLFML2B antibody (ab75355) at 1/500 dilution
Lane 1 : extracts from HUVEC cells Lane 2 : extracts from HUVEC cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 84 kDa Observed band size : 84 kDa Additional bands at : 48 kDa,65 kDa,86 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - Anti-OLFML2B antibody (ab75355)This image is courtesy of an Abreview submitted by Iram Pablo Rodriguez-Sanchez
ab75355 staining OLFML2B in Human retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with sodium borohydride and blocked with 5% milk for 2 hours at 25°C. Samples were incubated with primary antibody (undiluted) for 2 hours. A Cy3®-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.