製品の概要

  • 製品名Anti-OLFML2A antibody
    OLFML2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OLFML2A
  • 特異性ab75882 detects endogenous levels of total OLFML2A protein.
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthesized peptide derived from internal of human OLFML2A.

  • ポジティブ・コントロール
    • Extracts from RAW264.7 cells A549 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性OLFML2A contains one olfactomedin-like domain. Its exact function is unknown. There are three named isoforms produced by alternative splicing.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • FLJ00237 antibody
    • Olfactomedin like 2A antibody
    • Olfactomedin like protein 2A antibody
    • OLM2A_HUMAN antibody
    • Photomedin 1 antibody
    • PRO34319 antibody
    • UNQ9394/PRO34319 antibody
    see all

Anti-OLFML2A antibody 画像

  • ab75882 staining OLFML2A in Human retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 12 hours at 25°C. Samples were incubated with primary antibody (1/500) for 2 hours at 25°C. A Cy3®-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-OLFML2A antibody (ab75882) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells
    Lane 2 : extracts from RAW264.7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • ab75882, at a dilution of 1/500-1/1000, staining OLFML2A in A549 cells by Immunofluorescence with (+) or without (-) immunising peptide.

Anti-OLFML2A antibody (ab75882) 使用論文

ab75882 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Retina)
Specification Retina
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Prof. IRAM PABLO RODRIGUEZ-SANCHEZ

Verified customer

投稿 Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"