In Western Blot, ab85046 gave a positive signal in Human liver tissue lysate. In IHC, this antibody gave a positive signal in a Formalin-Fixed Paraffin-Embedded Human Prostate tissue section. In IF, this antibody gave a positive signal in T84 cell line.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).
An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion.
Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues.
Contains 1 olfactomedin-like domain.
The olfactomedin-like domain is involved in the interaction with cadherin.
Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus.
ab85046 stained T84 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85046 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - Anti-OLFM4 antibody (ab85046)
Anti-OLFM4 antibody (ab85046) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa Observed band size: 57 kDa
IHC image of OLFM4 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85046, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Quesada-Calvo F et al. OLFM4, KNG1 and Sec24C identified by proteomics and immunohistochemistry as potential markers of early colorectal cancer stages. Clin Proteomics14:9 (2017).
Read more (PubMed: 28344541) »
Wieck MM et al. Prolonged Absence of Mechanoluminal Stimulation in Human Intestine Alters the Transcriptome and Intestinal Stem Cell Niche. Cell Mol Gastroenterol Hepatol3:367-388.e1 (2017).
Read more (PubMed: 28462379) »