Anti-OLFM4 抗体 (ab85046)
Key features and details
- Rabbit polyclonal to OLFM4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-OLFM4 antibody
OLFM4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to OLFM4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- In Western Blot, ab85046 gave a positive signal in Human liver tissue lysate. ICC/IF: HT-29 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85046の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).
|
特記事項 |
---|
ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
-
機能
An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion. -
組織特異性
Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues. -
配列類似性
Contains 1 olfactomedin-like domain. -
ドメイン
The olfactomedin-like domain is involved in the interaction with cadherin. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 10562 Human
- Entrez Gene: 380924 Mouse
- Omim: 614061 Human
- SwissProt: Q6UX06 Human
- SwissProt: Q3UZZ4 Mouse
- Unigene: 508113 Human
- Unigene: 26456 Mouse
-
別名
- Antiapoptotic protein GW112 antibody
- bA209J19.1 antibody
- G-CSF-stimulated clone 1 protein antibody
see all
画像
-
ab85046 staining OLFM4 in HT29 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85046 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
Anti-OLFM4 antibody (ab85046) at 1 µg/ml + Human liver tissue lysate - total protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Additional bands at: 105 kDa (possible non-specific binding), 45 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab85046 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (17)
ab85046 は 17 報の論文で使用されています。
- Capeling MM et al. Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep 38:110379 (2022). PubMed: 35172130
- Nakanishi A et al. Effects of human induced pluripotent stem cell-derived intestinal organoids on colitis-model mice. Regen Ther 21:351-361 (2022). PubMed: 36161099
- Ohkuma R et al. High expression of olfactomedin-4 is correlated with chemoresistance and poor prognosis in pancreatic cancer. PLoS One 15:e0226707 (2020). PubMed: 31923206
- Wu A et al. Transmissible gastroenteritis virus targets Paneth cells to inhibit the self-renewal and differentiation of Lgr5 intestinal stem cells via Notch signaling. Cell Death Dis 11:40 (2020). PubMed: 31959773
- Zhang P et al. Dissecting the Single-Cell Transcriptome Network Underlying Gastric Premalignant Lesions and Early Gastric Cancer. Cell Rep 27:1934-1947.e5 (2019). PubMed: 31067475