Anti-OGT / O-Linked N-Acetylglucosamine Transferase 抗体 (ab97274)

製品の概要

  • 製品名
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    OGT / O-Linked N-Acetylglucosamine Transferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQ EPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYK ERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSAL QYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCV FNAQGEIWLAIHHFEKAVTLDPNFLD AYINLGNVLKEARI, corresponding to amino acids 1 - 240 of Human O-Linked N-Acetylglucosamine Transferase (NP_858059).

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 117 kDa.

ターゲット情報

  • 機能
    Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • 組織特異性
    Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • ドメイン
    The TPR repeat domain mediates recognition of protein substrates.
  • 翻訳後修飾
    Ubiquitinated, leading to its proteasomal degradation.
  • 細胞内局在
    Cytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

画像

  • ICC/IF image of ab97274 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97274 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab97274) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 117 kDa
    7.5% SDS PAGE

参考文献

ab97274 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (neuroblastoma)
Specification
neuroblastoma
Username

Abcam user community

Verified customer

投稿 Apr 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録