The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Protein modification; protein glycosylation.
Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.
The TPR repeat domain mediates recognition of protein substrates.
Ubiquitinated, leading to its proteasomal degradation.
An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
Primary incubation was 1 hour. Detected by chemiluminescence.
Moura CS et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res61:1290740 (2017).
Read more (PubMed: 28326005) »
Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One9:e83437 (2014).
Read more (PubMed: 24465380) »