Anti-OGT / O-Linked N-Acetylglucosamine Transferase 抗体 (ab59135)

製品の概要

  • 製品名
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    OGT / O-Linked N-Acetylglucosamine Transferase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • 特異性
    ab59135 is expected to recognize both reported isoforms of O-Linked N-Acetylglucosamine
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-YEHPKDLKLSDGR

    by a Cysteine residue linker, corresponding to amino acids 543-555 of Human O-Linked N-Acetylglucosamine

  • ポジティブ・コントロール
    • Rat or mouse pancreas cytoplasmic or nuclear lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: Antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
    • 組織特異性
      Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
    • パスウェイ
      Protein modification; protein glycosylation.
    • 配列類似性
      Belongs to the O-GlcNAc transferase family.
      Contains 13 TPR repeats.
    • ドメイン
      The TPR repeat domain mediates recognition of protein substrates.
    • 翻訳後修飾
      Ubiquitinated, leading to its proteasomal degradation.
    • 細胞内局在
      Cytoplasm. Nucleus. Mostly in the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ23071 antibody
      • GlcNAc transferase antibody
      • HRNT1 antibody
      • MGC22921 antibody
      • O GlcNAc antibody
      • O GlcNAc transferase p110 subunit antibody
      • O GlcNAc transferase subunit p110 antibody
      • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
      • O linked N acetylglucosamine (GlcNAc) transferase antibody
      • O linked N acetylglucosamine transferase 110 kDa subunit antibody
      • O-GlcNAc transferase subunit p110 antibody
      • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
      • ogt antibody
      • OGT1_HUMAN antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
      • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
      • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
      see all

    画像

    • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135) at 0.05 µg/ml + Rat pancreas lysate in RIPA buffer at 35 µg

      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    This product has been referenced in:
    • Moura CS  et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res 61:1290740 (2017). WB ; Rat . Read more (PubMed: 28326005) »
    • Moura CS  et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . Read more (PubMed: 24465380) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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