Anti-OGT / O-Linked N-Acetylglucosamine Transferase 抗体 (ab50271)

製品の概要

  • 製品名Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    OGT / O-Linked N-Acetylglucosamine Transferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    SQYGLPEDAIVYCNFNQ

    conjugated to KLH via an N-terminal added lysine residue, corresponding to amino acids 833-849 of Human O-Linked N-Acetylglucosamine

  • ポジティブ・コントロール
    • HeLa cell nuclear extract. HeLa cell lysate. HEK 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa). A 78kDa band may be seen in some preparations.
IP Use a concentration of 3 - 6 µg/ml.

ターゲット情報

  • 機能Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • 組織特異性Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • パスウェイProtein modification; protein glycosylation.
  • 配列類似性Belongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • ドメインThe TPR repeat domain mediates recognition of protein substrates.
  • 翻訳後修飾Ubiquitinated, leading to its proteasomal degradation.
  • 細胞内局在Cytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab50271) 使用論文

This product has been referenced in:
  • Cox EJ & Marsh SA Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart. Cardiovasc Diabetol 12:101 (2013). Read more (PubMed: 23835259) »
  • Kebede M  et al. Glucose activates free fatty acid receptor 1 gene transcription via phosphatidylinositol-3-kinase-dependent O-GlcNAcylation of pancreas-duodenum homeobox-1. Proc Natl Acad Sci U S A 109:2376-81 (2012). WB ; Human, Mouse . Read more (PubMed: 22308370) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cell)
Loading amount 20 µg
Specification Mouse embryonic stem cell
Gel Running Conditions Reduced Denaturing (4-12%, MOPS buffer)
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 May 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"