Anti-OGT / O-Linked N-Acetylglucosamine Transferase 抗体 (ab50270)

製品の概要

  • 製品名
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
    OGT / O-Linked N-Acetylglucosamine Transferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide:

    WEHYAAGNKPDHMI

    conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 1024-1037 of Human O-Linked N-Acetylglucosamine

  • ポジティブ・コントロール
    • HeLa cell nuclear extract and A549 human lung carcinoma cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).
ICC/IF 1/50 - 1/100. (Cells fixed with paraformaldehyde/triton).

ターゲット情報

  • 機能
    Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
  • 組織特異性
    Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.
  • ドメイン
    The TPR repeat domain mediates recognition of protein substrates.
  • 翻訳後修飾
    Ubiquitinated, leading to its proteasomal degradation.
  • 細胞内局在
    Cytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

画像

  • Cytoplasmic, and nuclear localization of O-Linked N-Acetylglucosamine in human lung carcinoma (A549) cells stained by ab50270. A. Working dilution of 1:100 B. Working dilution of 1:50

参考文献

This product has been referenced in:
  • Runager K  et al. Targeting O-glycosyltransferase (OGT) to promote healing of diabetic skin wounds. J Biol Chem 289:5462-6 (2014). WB ; Human . Read more (PubMed: 24398691) »
  • Li MD  et al. O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination. Cell Metab 17:303-10 (2013). Read more (PubMed: 23395176) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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