製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to OGR1
  • 特異性
    SPR1 antibody detects endogenous levels of total SPR1 protein.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow
  • 免疫原

    The antiserum was produced against synthesized peptide derived from internal of human SPR1.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.
ELISA 1/10000.

ターゲット情報

  • 機能
    Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer.
  • 組織特異性
    Found at low level in a wide range of tissues, but significantly expressed in lung, kidney, bone and nervous system.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • G protein coupled receptor 68 antibody
    • G-protein coupled receptor 68 antibody
    • GPR 12A antibody
    • GPR 68 antibody
    • GPR12A antibody
    • Gpr68 antibody
    • MGC111379 antibody
    • MGC156983 antibody
    • OGR 1 antibody
    • OGR-1 antibody
    • OGR1 antibody
    • OGR1_HUMAN antibody
    • Ovarian cancer G protein coupled receptor 1 antibody
    • Ovarian cancer G-protein coupled receptor 1 antibody
    • Sphingosylphosphorylcholine receptor antibody
    see all

画像

  • All lanes : Anti-OGR1 antibody (ab72500) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 41 kDa

プロトコール

参考文献

ab72500 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization
No
Specification
Lung
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 08 2017

Thank you for your reply and for submitting your Abreview. I have activated your discount code and it can now be used to get a free primary antibody on a future order. Please let me know if you need any additional information or assistance.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Spleen)
Loading amount
25 µg
Specification
Spleen
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Aug 13 2012

DISCOUNT CODE: ***
Expiration date: October 12, 2012
I am very pleased to hear you would like to accept our offer and test ab72500 in mouse. This code will give you: 1 free primary antibody of your choice before the expiration date. To redeem...

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Thank you very much for your interest in our GPR68 antibodies.
While we do not provide free or trial sized samples of any of our products, we do have a program for testing in untested species or applications. I can offer a discount off a future pu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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