製品の概要

  • 製品名Anti-Oct4 antibody [PR-2H9]
    Oct4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PR-2H9] to Oct4
  • アプリケーション適用あり: WB, Indirect ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human Oct4 aa 1-212.

  • ポジティブ・コントロール
    • Recombinant Oct4 protein, HEK293-Oct-4 transfectant cell lysate, NTERA-2 cell lysate, and 2102 cell lysate.
  • 特記事項

    This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 39 kDa.
Indirect ELISA 1/200 - 1/1000.

ターゲット情報

  • 機能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 組織特異性Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • 配列類似性Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 発生段階Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • ドメインThe POU-specific domain mediates interaction with PKM2.
  • 翻訳後修飾Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • 細胞内局在Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Anti-Oct4 antibody [PR-2H9] 画像

  • Indirect ELISA detection of immobilized Oct4 (full length) with titrated amounts of ab95854. No binding is seen to irrelevant recombinant proteins Nanog or SOX2.

  • Lane 1 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 0.1 µg/ml
    Lanes 2 - 6 : Anti-Oct4 antibody [PR-2H9] (ab95854) at 1 µg/ml

    Lane 1 : Recombinant Oct-4 protein at 0.25 µg
    Lane 2 : Recombinant Oct-4 protein at 0.25 µg
    Lane 3 : Untransfected HEK293 lysate at 20 µg
    Lane 4 : HEK293-Oct-4 transfectant cell lysate at 20 µg
    Lane 5 : NTERA-2 cell lysate at 20 µg
    Lane 6 : 2102 cell lysate at 20 µg


    Predicted band size : 39 kDa

Anti-Oct4 antibody [PR-2H9] (ab95854) 使用論文

ab95854 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"