Anti-Oct4 抗体 [NRG1.1], prediluted (ab73099)

製品の概要

  • 製品名Anti-Oct4 antibody [NRG1.1], prediluted
    Oct4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NRG1.1] to Oct4, prediluted
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Oct4.

  • ポジティブ・コントロール
    • Human seminoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報IHC-P: Use neat for 30 minutes at room temperature.
    Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • 組織特異性Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
    • 配列類似性Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • 発生段階Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
    • ドメインThe POU-specific domain mediates interaction with PKM2.
    • 翻訳後修飾Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
    • 細胞内局在Nucleus. Expressed in a diffuse and slightly punctuate pattern.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Octamer binding transcription factor 4 antibody
      • MGC22487 antibody
      • Oct 3 antibody
      • Oct 4 antibody
      • Oct-3 antibody
      • Oct-4 antibody
      • OCT3 antibody
      • Oct4 antibody
      • Octamer binding protein 3 antibody
      • Octamer binding protein 4 antibody
      • Octamer binding transcription factor 3 antibody
      • Octamer-binding protein 3 antibody
      • Octamer-binding protein 4 antibody
      • Octamer-binding transcription factor 3 antibody
      • OTF 3 antibody
      • OTF 4 antibody
      • OTF-3 antibody
      • OTF3 antibody
      • OTF4 antibody
      • PO5F1_HUMAN antibody
      • POU class 5 homeobox 1 antibody
      • POU domain class 5 transcription factor 1 antibody
      • POU domain transcription factor OCT4 antibody
      • POU domain, class 5, transcription factor 1 antibody
      • POU-type homeodomain-containing DNA-binding protein antibody
      • POU5F1 antibody
      see all

    Anti-Oct4 antibody [NRG1.1], prediluted 画像

    • ab73099 staining Oct4 in human seminomatous tumor by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Anti-Oct4 antibody [NRG1.1], prediluted (ab73099) 使用論文

    ab73099 has not yet been referenced specifically in any publications.

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    Thank you for contacting us.

    We recommend using Human seminoma tissue as positive control with this product. We currently have Testis tumor (human): seminoma tissue slides available on our catalog (http://www.abcam.com/testis-tumor-human-s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"