Anti-Oct4 抗体 [EPR2054] (ab109183)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2054] to Oct4
- Suitable for: ChIC/CUT&RUN-seq, WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Oct4 antibody [EPR2054]
Oct4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2054] to Oct4 -
由来種
Rabbit -
アプリケーション
適用あり: ChIC/CUT&RUN-seq, WB, IP, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: NCCIT and HeLa whole cell lysate (ab150035). IHC-P: Human seminoma and ovarian dysgerminoma tissue. IP: NCCIT whole cell lysate. ChIC/CUT&RUN-Seq: NCCIT cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 5.00 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2054 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109183の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5 µg |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5 µg |
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. -
組織特異性
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. -
配列類似性
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
発生段階
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. -
ドメイン
The POU-specific domain mediates interaction with PKM2. -
翻訳後修飾
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. -
細胞内局在
Nucleus. Expressed in a diffuse and slightly punctuate pattern. - Information by UniProt
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参照データベース
- Entrez Gene: 5460 Human
- Omim: 164177 Human
- SwissProt: Q01860 Human
- Unigene: 249184 Human
- Unigene: 632482 Human
- Unigene: 646545 Human
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別名
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
see all
画像
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ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/µL, 2 x 10^5 NCCIT (Human pluripotent embryonic carcinoma cell line) cells and 5 µg of ab109183 [EPR2054]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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Anti-Oct4 antibody [EPR2054] (ab109183) at 0.06 µg/ml (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human seminoma tissue sections labeling Oct4 - with Purified ab109183 at 1:600 dilution (1 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
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All lanes : Anti-Oct4 antibody [EPR2054] (ab109183) at 1/1000 dilution (Unpurified)
Lane 1 : Seminoma lysate
Lane 2 : NCCIT cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
Unpurified ab109183, at 1:500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 at 1/50 immunoprecipitating Oct4 in NCCIT (human pluripotent embryonal carcinoma) whole cell lysate observed at 39 KDa (lanes 1 and 2).
Lane 1 (input): NCCIT whole cell lysate, 10μg
Lane 2 (+): ab109183 + NCCIT whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109183 in NCCIT whole cell lysate
For western blotting, ab109183 at 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Unpurified ab109183 showing negative staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 showing positive staining in Seminoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 showing positive staining in Fetal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 showing negative staining in Hepatocellular carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109183 showing positive staining in Embryonal carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab109183 は 39 報の論文で使用されています。
- Shen P et al. LncRNA CASC15 regulates breast cancer cell stemness via the miR-654-5p/MEF2D axis. J Biochem Mol Toxicol 36:e23023 (2022). PubMed: 35235236
- Salah M et al. Reactive oxygen species-inducing titanium peroxide nanoparticles as promising radiosensitizers for eliminating pancreatic cancer stem cells. J Exp Clin Cancer Res 41:146 (2022). PubMed: 35428310
- Li H et al. Chelerythrine Chloride Inhibits Stemness of Melanoma Cancer Stem-Like Cells (CSCs) Potentially via Inducing Reactive Oxygen Species and Causing Mitochondria Dysfunction. Comput Math Methods Med 2022:4000733 (2022). PubMed: 35761835
- Kaewpiboon C et al. Formoxanthone C Inhibits Malignant Tumor Phenotypes of Human A549 Multidrug Resistant-cancer Cells through Signal Transducer and Activator of Transcription 1-Histone Deacetylase 4 Signaling. J Cancer Prev 27:112-121 (2022). PubMed: 35864853
- Li L et al. Knockdown of miR-92a suppresses the stemness of colorectal cancer cells via mediating SOCS3. Bioengineered 13:5613-5624 (2022). WB ; Human . PubMed: 35184640