製品の概要

  • 製品名Anti-Oct4 antibody
    Oct4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Oct4
  • アプリケーション適用あり: Sandwich ELISA, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus Monkey, Macaque Monkey
  • 免疫原

    Recombinant fragment corresponding to Human Oct4 aa 81-164.
    Sequence:

    WFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYG SPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSN

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Oct4 (ab53028).

For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Oct4 (ab53028) as Detection.

WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 組織特異性Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • 配列類似性Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 発生段階Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • ドメインThe POU-specific domain mediates interaction with PKM2.
  • 翻訳後修飾Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • 細胞内局在Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Anti-Oct4 antibody 画像

  • Standard Curve for Oct4; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal to Oct4 (ab77259) at 5 ug/ml and Detector Antibody Rabbit polyclonal to Oct-4 (ab53028) at 0.5 ug/ml.

  • Anti-Oct4 antibody (ab77259) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Anti-Oct4 antibody (ab77259) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

プロトコール

Anti-Oct4 antibody (ab77259) 使用論文

ab77259 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

I have identified a pair of antibodies that have been tested and guaranteed to work in sandwich ELISA against the human Oct4 protein, a protein involved in stem cell self-renewal. Links to the antibodies are giv...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"