Anti-Oct4 抗体 (ab137427)
Key features and details
- Rabbit polyclonal to Oct4
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Oct4 antibody
Oct4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Oct4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Cow, Cat, Pig, Monkey -
免疫原
Recombinant fragment corresponding to Human Oct4 aa 1-182.
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ポジティブ・コントロール
- Recombinant human Oct4 protein (ab134876) can be used as a positive control in WB. HeLa, Human embryonic stem cells (ESC) and Mouse ESC whole cell lysates; HeLa nuclear cell lysate; Human embryonic stem cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Affinity Purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137427の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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Flow Cyt |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Flow Cyt
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. -
組織特異性
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. -
配列類似性
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
発生段階
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. -
ドメイン
The POU-specific domain mediates interaction with PKM2. -
翻訳後修飾
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. -
細胞内局在
Nucleus. Expressed in a diffuse and slightly punctuate pattern. - Information by UniProt
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参照データベース
- Entrez Gene: 282316 Cow
- Entrez Gene: 5460 Human
- Entrez Gene: 18999 Mouse
- Entrez Gene: 100127461 Pig
- Entrez Gene: 100101567 Rabbit
- Omim: 164177 Human
- SwissProt: O97552 Cow
- SwissProt: Q01860 Human
see all -
別名
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
see all
画像
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Anti-Oct4 antibody (ab137427) at 1/5000 dilution + Mouse Embryonic Stem Cell whole cell lysate
at 20 µg
Predicted band size: 38 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:400 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:200 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:100 dilution. Lower image shows cells co-stained with DAPI.
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Anti-Oct4 antibody (ab137427) at 1/1000 dilution + HeLa nuclear cell lysate at 20 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Anti-Oct4 antibody (ab137427) at 1/1000 dilution + Human Embryonic Stem Cell whole cell lysate at 20 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Flow cytometric analysis of Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:50 dilution (green) or rabbit IgG (black).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab137427 は 14 報の論文で使用されています。
- Arcuri S et al. Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts. Front Vet Sci 8:706106 (2021). PubMed: 34350230
- Zhao X et al. TNF-a promotes the malignant transformation of intestinal stem cells through the NF-?B and Wnt/ß-catenin signaling pathways. Oncol Rep 44:577-588 (2020). PubMed: 32627006
- Flamini MA et al. Key characteristics of the ovary and uterus for reproduction with particular reference to poly ovulation in the plains viscacha (Lagostomus maximus, chinchillidae). Theriogenology 142:184-195 (2020). PubMed: 31604179
- Dong W et al. SATB2 knockdown decreases hypoxia-induced autophagy and stemness in oral squamous cell carcinoma. Oncol Lett 20:794-802 (2020). PubMed: 32566006
- Lima DBC et al. Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret (Mustela Nigripes). Animals (Basel) 10:N/A (2020). PubMed: 33066219