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RabMAb

Anti-Oct-2 抗体 [EPR542] (ab92458)

製品の概要

  • 製品名
    Anti-Oct-2 antibody [EPR542]
    Oct-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR542] to Oct-2
  • アプリケーション
    適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Oct-2 aa 150-250.
    Database link: P09086

  • ポジティブ・コントロール
    • Daudi cell lysate and Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Oct-2 antibody (HRP) [EPR542] (ab200602)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR542
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC
Flow Cyt
  • 追加情報
    Flow Cyt: 1/50.
    ICC: 1/250 - 1/500.
    IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
    WB: 1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
    • 組織特異性
      Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
    • 配列類似性
      Belongs to the POU transcription factor family. Class-2 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • 細胞内局在
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    画像

    • ab92458, at 1:250 staining Oct-2 in Human tonsil paraffin embeeded section by immunohistochemistry.

    • Anti-Oct-2 antibody [EPR542] (ab92458) at 1/1000 dilution + Daudi lysate at 10 µg

      Secondary
      HRP conjugated goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing Raji cells stained with ab92458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92458, 1:50) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献

    ab92458 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for your enquiry.

    I can confirm that this antibody (ab92458) has been tested for these applications (Flow, ICC and WB).

    If you need any further assistance in the future, please do not hesitate to contact me.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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