製品の概要

  • 製品名Anti-Oct-1 antibody
    Oct-1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Oct-1
  • 特異性This antibody does not cross react with OCT4 or OCT-2.
  • アプリケーション適用あり: IHC-P, Dot blot, WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Oct-1 (C terminal).
    Database link: P14859

  • ポジティブ・コントロール
    • Jurkat cell lysate. Mouse kidney lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2 with antibody stabiliser.
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
Dot blot 1/1000.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

ターゲット情報

  • 機能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
  • 組織特異性Ubiquitous. Isoform 2 is lymphocyte-specific.
  • 配列類似性Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 翻訳後修飾Phosphorylated by PRKDC.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ42836 antibody
    • NF A1 antibody
    • NF-A1 antibody
    • Oct 1 antibody
    • Oct 1B antibody
    • Oct-1 antibody
    • OCT1 antibody
    • Octamer binding protein 1 antibody
    • Octamer binding transcription factor 1 antibody
    • Octamer-binding protein 1 antibody
    • Octamer-binding transcription factor 1 antibody
    • OTF 1 antibody
    • OTF-1 antibody
    • OTF1 antibody
    • OTTHUMP00000032348 antibody
    • OTTHUMP00000032350 antibody
    • OTTHUMP00000032351 antibody
    • PO21 antibody
    • PO2F1 antibody
    • PO2F1_HUMAN antibody
    • POU class 2 homeobox 1 antibody
    • POU domain class 2 transcription factor 1 antibody
    • POU domain, class 2, transcription factor 1 antibody
    • POU2F1 antibody
    see all

Anti-Oct-1 antibody 画像

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded murine neural tissue sections, labelling Oct-1 with ab66132 at a dilution of 1/250 incubated for 90 minutes at 25°C in PBS plus 1x Casein. Heat mediated antigen retrival was with 10mM citrate at pH 6.0. Blocking was with 10% serum incubated for 30 minutes at 25°C. Secondary used was a Goat anti-Rabbit polyclonal biotin conjugate at 1/200.

    See Abreview

  • 1µg Oct-1 peptide (A) on a nitrocellulose membrane followed by ab66132 at 1:1000 dilution. The labels B and C refer to 1 µg Oct-2 peptide and Oct4 peptide respectively.

  • All lanes : Anti-Oct-1 antibody (ab66132) at 1/1000 dilution

    Lane 1 : Murine cardiomyocyte whole cell lysates
    Lane 2 : Murine cardiomyocyte whole cell lysates
    Lane 3 : Murine cardiomyocyte whole cell lysates
    Lane 4 : Murine cardiomyocyte whole cell lysates

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Rabbit polyclonal HRP conjugate at 1/5000 dilution

    Predicted band size : 77 kDa
    Additional bands at : 50 kDa (possible non-specific binding).

    Image is courtesy of an anonymous AbReview.

    From left to right: Molecular weight marker ladder, lane1, 2, 3, 4. Blocking was done with 5% milk incubated for 1 hour at room temperature.

    See Abreview

  • All lanes : Anti-Oct-1 antibody (ab66132) at 1/500 dilution

    Lane 1 : mouse kidney tissue lysate
    Lane 2 : mouse kidney tissue lysate with immunising peptide

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 34 kDa,90 kDa. We are unsure as to the identity of these extra bands.

Anti-Oct-1 antibody (ab66132) 使用論文

ab66132 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions Reduced Denaturing (10% PAGE)
Loading amount 25 µg
Specification Cardiomyocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2015

Expiration date: 30-November-2012

I am very pleased to hear you would like to accept our offer and test ab15112, ab51363 and ab66132in Flow Cytometry. These codes will give you:3 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the...

Read More

Thank you for contacting us.

Could you let me know the sample type, species and application e.g. WB, IHC-P customer is interested in?

Please note the abreview discount code is valid only for untested species and applications. When se...

Read More

Thank you for your interest.

We have some antibodies against Oct1 but currently none of them have been tested in Flow cytometry. These products have been characterized in IHC and WB.

http://www.abcam.com/ab15112: Anti-Oct1 antibody ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"