The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 6 kDa).
Use at an assay dependent concentration.
Use a concentration of 2.5 µg/ml.
High expression in placenta, skeletal muscle, kidney and pancreas tissues. Absent or very faint expression in heart, brain, lung and liver. Expressed during adipogenic differentiation of mesenchymal stem cells (at protein level).