製品の概要

  • 製品名Anti-OBFC2B antibody
    OBFC2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OBFC2B
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 161 and 211 of human OBFC2B (NP_076973.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues.

ターゲット情報

  • 関連性Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. OBFC2B plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
  • 細胞内局在Nuclear. Localizes to nuclear foci following DNA damage.
  • 参照データベース
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Anti-OBFC2B antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling OBFC2B with ab85752 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-OBFC2B antibody (ab85752) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 23 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human OBFC2B by Western Blot of Immunprecipitate.
    All lanes: ab85752 at 1µg/ml detecting by WB OBFC2B in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 5 seconds.

Anti-OBFC2B antibody (ab85752) 使用論文

This product has been referenced in:
  • Chen ZW  et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res 42:11560-9 (2014). Read more (PubMed: 25249620) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"