Anti-hSSB1 抗体 (ab85752)
Key features and details
- Rabbit polyclonal to hSSB1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-hSSB1 antibody -
製品の詳細
Rabbit polyclonal to hSSB1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human hSSB1 aa 161-211.
Database link: NP_076973.1 -
ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85752の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues. |
特記事項 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues. |
ターゲット情報
-
関連性
Single-stranded DNA (ssDNA)-binding proteins, such as hSSB1, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. hSSB1 plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins. -
細胞内局在
Nuclear. Localizes to nuclear foci following DNA damage. -
参照データベース
- Entrez Gene: 79035 Human
- Entrez Gene: 69917 Mouse
- Entrez Gene: 362813 Rat
- Omim: 612104 Human
- SwissProt: Q9BQ15 Human
- SwissProt: Q8R2Y9 Mouse
- SwissProt: Q3SWT1 Rat
-
別名
- 2610036N15Rik antibody
- hSSB1 antibody
- LP3587 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling hSSB1 with ab85752 at 1/1000 (1µg/ml). Detection: DAB.
-
All lanes : Anti-hSSB1 antibody (ab85752) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 23 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human hSSB1 by Western Blot of Immunprecipitate.
All lanes: ab85752 at 1µg/ml detecting by WB hSSB1 in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 5 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab85752 は 1 報の論文で使用されています。
- Chen ZW et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res 42:11560-9 (2014). PubMed: 25249620