製品の概要

  • 製品名
    Obestatin
  • 製品の詳細
    Endogenous peptide involved in feeding
  • 生理活性の詳細
    Obestatin, a peptide encoded by the ghrelin gene that suppresses food intake. Binds to the orphan G-protein-coupled receptor GPR39.
  • 精製度
    > 95%

製品の特性

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  • ab7963 staining c-Fos in NIH 3T3 cells treated with obestatin (ab120071), by ICC/IF. Increase in c-Fos expression correlates with increased concentration of obestatin, as described in literature.
    The cells were incubated at 37°C for 3h in media containing different concentrations of ab120071 (obestatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7963 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

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