製品の概要

  • 製品名
    Anti-OATP2 antibody [MDQ]
    OATP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MDQ] to OATP2
  • 特異性
    Ab15442 will also cross react with OATP8.
  • アプリケーション
    適用あり: WB, IHC-Fr, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human OATP2 aa 1-23.
    Sequence:

    MDQNQHLNKTAEAQPSENKKTRY

  • ポジティブ・コントロール
    • Human liver tissue slides.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-Fr 1/20.
ICC/IF 1/20.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Mediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. May play an important role in the clearance of bile acids and organic anions from the liver.
  • 組織特異性
    Highly expressed in liver. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.
  • 配列類似性
    Belongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • 細胞内局在
    Basolateral cell membrane. Detected in basolateral membranes of hepatocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Liver-specific organic anion transporter 1 antibody
    • LST-1 antibody
    • LST1 antibody
    • OATP-2 antibody
    • OATP-C antibody
    • OATP1B1 antibody
    • OATPC antibody
    • SLC21A6 antibody
    • SLCO1B1 antibody
    • SO1B1_HUMAN antibody
    • Sodium-independent organic anion-transporting polypeptide 2 antibody
    • Solute carrier family 21 member 6 antibody
    • Solute carrier organic anion transporter family member 1B1 antibody
    see all

画像

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling OATP2 (green) with ab15542 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

参考文献

This product has been referenced in:
  • Imada S  et al. Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter. Surg Case Rep 2:89 (2016). Read more (PubMed: 27589984) »
  • Peng JF  et al. Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid. Biomol Ther (Seoul) 23:400-6 (2015). WB . Read more (PubMed: 26336578) »

See all 3 Publications for this product

レビューと Q&A



Any products that require an MSDS form will have a link on the product's online datasheet to the data. It will be a PDF document link labeled 'SDS'. Please see attached image for example of what to look for on the datasheet. If no 'SDS' link ...

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Application
Western blot
Sample
Human Cell lysate - whole cell (human HEK293 cells over-expressing)
Loading amount
150 µg
Specification
human HEK293 cells over-expressing
Gel Running Conditions
Reduced Denaturing (denature at 70C for 10mins, 7.5% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Sep 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (transiently transfected HeLa cells)
Loading amount
100000 cells
Specification
transiently transfected HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Dec 19 2006

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read through your technical questionaire and I have a few comments. OATP2 antibody [MDQ] (ab15442) has been tested for its applicat...

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Thank you for your enquiry. Both antibodies were tested in IP using human liver samples (~20-50 ug) and about 1 ug/ml of antibody used. For IF, human liver and transfected HEK293 whole cell lysates were used. Ab15442 was used at a dilution of 1:20. The...

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Thank you for bringing this to our attention. The immunogen sequence is from OATP2, however this product will also recognise OATP8. The data sheet has now been updated to reflect this. Please let me know if you spot any further problems. I apologize fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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