製品の概要

  • 製品名
    Anti-OASL antibody - Carboxyterminal end
    OASL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OASL - Carboxyterminal end
  • 特異性
    This antibody is specific for OASL (C term).
  • アプリケーション
    適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human OASL.

  • ポジティブ・コントロール
    • HL60 lysate and breast carcinoma tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.
  • 組織特異性
    Expressed in most tissues, with the highest levels in primary blood Leukocytes and other hematopoietic system tissues, colon, stomach and to some extent in testis.
  • 配列類似性
    Belongs to the 2-5A synthase family.
    Contains 2 ubiquitin-like domains.
  • ドメイン
    The ubiquitin-like domains are essential for its antiviral activity.
  • 細胞内局在
    Cytoplasm and Nucleus > nucleolus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2' 5' oligoadenylate synthetase like antibody
    • 2''-5''-OAS-related protein antibody
    • 2''-5''-OAS-RP antibody
    • 2''-5''-oligoadenylate synthase-like protein antibody
    • 2'-5' oligoadenylate synthetase-like 2 antibody
    • 2'-5'-oligoadenylate synthetase-like antibody
    • 59 kDa 2' 5' oligoadenylate synthetase like protein antibody
    • 59 kDa 2''-5''-oligoadenylate synthase-like protein antibody
    • 59 kDa 2'-5'-oligoadenylate synthetase-like protein antibody
    • M1204 antibody
    • Mmu-OASL antibody
    • OASL antibody
    • OASL_HUMAN antibody
    • Oasl2 antibody
    • p59 OASL antibody
    • p59OASL antibody
    • THYROID HORMONE RECEPTOR INTERACTOR 14 antibody
    • Thyroid receptor interacting protein 14 antibody
    • Thyroid receptor-interacting protein 14 antibody
    • TR-interacting protein 14 antibody
    • TRIP-14 antibody
    • TRIP14 antibody
    see all

画像

  • ab38325 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38325 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-OASL antibody - Carboxyterminal end (ab38325) at 1/100 dilution + HL60 lysate

    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38325 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

参考文献

This product has been referenced in:
  • Kroeker AL  et al. Response of primary human airway epithelial cells to influenza infection: a quantitative proteomic study. J Proteome Res 11:4132-46 (2012). WB . Read more (PubMed: 22694362) »

See 1 Publication for this product

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