製品の概要

  • 製品名Anti-OASL antibody - Carboxyterminal end
    OASL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OASL - Carboxyterminal end
  • 特異性This antibody is specific for OASL (C term).
  • アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human OASL.

  • ポジティブ・コントロール
    • HL60 lysate and breast carcinoma tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab38325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.
  • 組織特異性Expressed in most tissues, with the highest levels in primary blood Leukocytes and other hematopoietic system tissues, colon, stomach and to some extent in testis.
  • 配列類似性Belongs to the 2-5A synthase family.
    Contains 2 ubiquitin-like domains.
  • ドメインThe ubiquitin-like domains are essential for its antiviral activity.
  • 細胞内局在Cytoplasm and Nucleus > nucleolus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2' 5' oligoadenylate synthetase like antibody
    • 2''-5''-OAS-related protein antibody
    • 2''-5''-OAS-RP antibody
    • 2''-5''-oligoadenylate synthase-like protein antibody
    • 2'-5' oligoadenylate synthetase-like 2 antibody
    • 2'-5'-oligoadenylate synthetase-like antibody
    • 59 kDa 2' 5' oligoadenylate synthetase like protein antibody
    • 59 kDa 2''-5''-oligoadenylate synthase-like protein antibody
    • 59 kDa 2'-5'-oligoadenylate synthetase-like protein antibody
    • M1204 antibody
    • Mmu-OASL antibody
    • OASL antibody
    • OASL_HUMAN antibody
    • Oasl2 antibody
    • p59 OASL antibody
    • p59OASL antibody
    • THYROID HORMONE RECEPTOR INTERACTOR 14 antibody
    • Thyroid receptor interacting protein 14 antibody
    • Thyroid receptor-interacting protein 14 antibody
    • TR-interacting protein 14 antibody
    • TRIP-14 antibody
    • TRIP14 antibody
    see all

Anti-OASL antibody - Carboxyterminal end 画像

  • ab38325 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38325 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-OASL antibody - Carboxyterminal end (ab38325) at 1/100 dilution + HL60 lysate

    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38325 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-OASL antibody - Carboxyterminal end (ab38325) 使用論文

This product has been referenced in:
  • Kroeker AL  et al. Response of primary human airway epithelial cells to influenza infection: a quantitative proteomic study. J Proteome Res 11:4132-46 (2012). WB . Read more (PubMed: 22694362) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab38325.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"