製品の概要

  • 製品名
    Anti-NY-REN-50 antigen antibody
  • 製品の詳細
    Rabbit polyclonal to NY-REN-50 antigen
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse
  • 免疫原

    Synthetic peptide: CSQETLEINN DKVAESLGIT EFLRKKEIHP DNLGPKHLSR DMDGEQLEGA, corresponding to a region within internal sequence amino acids 541-590 of Human NY-REN-50 antigen

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 0.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 配列類似性
    Contains 1 C2H2-type zinc finger.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C14orf131 antibody
    • C14orf131 protein antibody
    • Chromosome 14 open reading frame 131 antibody
    • FLJ11132 antibody
    • Hypothetical protein LOC55778 antibody
    • Renal carcinoma antigen NY-REN-50 antibody
    • Zinc finger protein 839 antibody
    • Zinc finger protein C14orf131 antibody
    • ZN839_HUMAN antibody
    • ZNF839 antibody
    see all

画像

  • Anti-NY-REN-50 antigen antibody (ab66345) at 0.4 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab66345 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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