製品の概要

  • 製品名Anti-NXF5 antibody
    NXF5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NXF5
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Dog
  • 免疫原

    Synthetic peptide: TEKAPKVKTL NLSKNKLESA WELGKVKGLK LEELWLEGNP LCSTFSDQSA, corresponding to amino acids 181-230 of Human NXF5

  • ポジティブ・コントロール
    • Jurkat cell lysate; Paraffin-embedded human kidney tissue, Paraffin-embedded human intestine tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性NXF5 is a member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for the NXF5 gene.
  • 細胞内局在Cytoplasmic and Nuclear. Mainly localized in the cytoplasm particularly in the cell body and neurites of hippocampal neurons. Nuclear localization is also observed. Not detected at nuclear rim.
  • 参照データベース
  • 別名
    • Nuclear RNA export factor 5 antibody
    • TAP like protein 1 antibody
    • TAPL 1 antibody
    • TAPL1 antibody

Anti-NXF5 antibody 画像

  • Anti-NXF5 antibody (ab50954) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Tissue: paraffin-embedded human Kidney
    Cells with positive label: Epithelial cells of renal tubule (indicated with arrows)
    Antibody concentration 4 - 8 µg/ml
    Magnification: 400X
  • Tissue: paraffin-embedded human Intestine
    Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows)
    Antibody concentration: 4 - 8 µg/ml
    Magnification: 400X

Anti-NXF5 antibody (ab50954) 使用論文

ab50954 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50954.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"