製品の概要

  • 製品名Anti-NXF3 antibody
    NXF3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NXF3
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to a region of Human NXF3.

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

ターゲット情報

  • 機能May function as a tissue-specific nuclear mRNA export factor.
  • 組織特異性Expressed at high level in testis and at low level in a small number of tissues.
  • 配列類似性Belongs to the NXF family.
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • ドメインLacks C-terminal domain that mediates direct interactions with nucleoporins.
    Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain.
    The RNA-binding domain is a non-canonical RNP-type domain.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Nuclear RNA export factor 3 antibody
    • NXF3 antibody
    • NXF3_HUMAN antibody
    • OTTHUMP00000023726 antibody
    • OTTHUMP00000023727 antibody
    • TAP like protein 3 antibody
    • TAP-like protein 3 antibody
    • TAPL 3 antibody
    • TAPL-3 antibody
    • TAPL3 antibody
    see all

Anti-NXF3 antibody 画像



  • Predicted band size : 60 kDa
    Western blot analysis of HeLa cystosolic (lane 1) and nuclear (lane 2) lysates (7µg/lane) labeling NXF3 with ab76430 at 1:300. A HRP-conjugated anti-rabbit (1:500) was used as the secondary antibody.
  • Anti-NXF3 antibody (ab76430) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%

Anti-NXF3 antibody (ab76430) 使用論文

ab76430 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab76430.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"