The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in western blotting.
Use a concentration of 5 µg/ml. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125 °C for 4min and under 90 °C for 45min, cool sample subsequently.
機能May function as a tissue-specific nuclear mRNA export factor.
組織特異性Expressed at high level in testis and at low level in a small number of tissues.
配列類似性Belongs to the NXF family. Contains 1 NTF2 domain. Contains 1 RRM (RNA recognition motif) domain.
ドメインLacks C-terminal domain that mediates direct interactions with nucleoporins. Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain. The RNA-binding domain is a non-canonical RNP-type domain.
細胞内局在Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.