Anti-NUP98 抗体 [2H10] - Nuclear Pore Marker (ab50610)
Key features and details
- Rat monoclonal [2H10] to NUP98 - Nuclear Pore Marker
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2c
製品の概要
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製品名
Anti-NUP98 antibody [2H10] - Nuclear Pore Marker
NUP98 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [2H10] to NUP98 - Nuclear Pore Marker -
由来種
Rat -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Recombinant fragment corresponding to Human NUP98 aa 1-466.
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ポジティブ・コントロール
- WB: HeLa nuclear lysate. Jurkat, HeLa, COS-7, NIH/3T3 and SH-SY5Y cell lysate. ICC/IF: HeLa and NIH/3T3 cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
2H10 -
ミエローマ
Sp2 -
アイソタイプ
IgG2c -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50610の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000. Detects a band of approximately 98 kDa.
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ICC/IF | (1) |
Use at an assay dependent concentration.
Customers have reported that Paraformaldehyde/Triton x-100 fixation provides better results, with sharp, regularly punctuate perinuclear signals. In MetOH fixed cells, the signal intensity can be somehwat lower and fuzzier and that single nucleoporin dots can be harder to distinguish around nuclear chromatin. Please see images below. |
特記事項 |
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WB
1/1000. Detects a band of approximately 98 kDa. |
ICC/IF
Use at an assay dependent concentration. Customers have reported that Paraformaldehyde/Triton x-100 fixation provides better results, with sharp, regularly punctuate perinuclear signals. In MetOH fixed cells, the signal intensity can be somehwat lower and fuzzier and that single nucleoporin dots can be harder to distinguish around nuclear chromatin. Please see images below. |
ターゲット情報
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機能
Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. -
関連疾患
Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
配列類似性
Belongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain. -
ドメイン
Contains G-L-F-G repeats. -
翻訳後修飾
Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. -
細胞内局在
Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket. - Information by UniProt
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参照データベース
- Entrez Gene: 4928 Human
- Entrez Gene: 269966 Mouse
- Entrez Gene: 81738 Rat
- Omim: 601021 Human
- SwissProt: P52948 Human
- SwissProt: Q6PFD9 Mouse
- SwissProt: P49793 Rat
- Unigene: 524750 Human
see all -
別名
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
see all
画像
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Lentiviral vector-encoded shRNAs achieve efficient knock-down of human nucleoporins and have negligible cytotoxic or cytostatic effects.
HeLa cells (4×106) were transduced with lentiviral vectors (MOI 50) encoding shRNAs specific for the indicated nucleoporins and used at 2 days p.t for Nup153 shRNA and 5 days p.t for all others.
(Panel B) Subcellular localisation of nuclear pore components upon nucleoporin knock-down was tested by confocal fluorescence microscopy of LV- (Control) and LV-shRNA transduced cells using specific anti-Nup antibodies. Images were acquired on the same day with the same conditions and are representative of two independent experiments.
This image was generated using the ascites version of the product.
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All lanes : Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 7 : P19 cell lysate
Lane 8 : NRK cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.This image was generated using the ascites version of the product.
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ab50610 (1/100) staining NUP98 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (green).
Cells were fixed with paraformaldehyde/Triton X-100 [10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature] or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).
This image was generated using the ascites version of the product.
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ab50610 (1/100) staining NUP98 in NIH/3T3 (Mouse embryo fibroblast cell line) cells (green).
Cells were fixed with paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature) or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).
This image was generated using the ascites version of the product.
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Paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for NUP98 (green) using ab50610 at 1/200 dilution in ICC/IF, followed by Donkey Anti-Rat Alexa Fluor® 488.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab50610 は 25 報の論文で使用されています。
- Gleixner AM et al. NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nat Commun 13:3380 (2022). PubMed: 35697676
- Uhlírová J et al. Nucleoporin TPR Affects C2C12 Myogenic Differentiation via Regulation of Myh4 Expression. Cells 10:N/A (2021). PubMed: 34063931
- Shiimori M et al. Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. iScience 24:102660 (2021). PubMed: 34169237
- Lin YC et al. Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. Nat Neurosci 24:1077-1088 (2021). PubMed: 34059832
- Cavazza T et al. Parental genome unification is highly error-prone in mammalian embryos. Cell 184:2860-2877.e22 (2021). PubMed: 33964210