Rabbit polyclonal to NUP88
Mouse, Rat, Cow, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NUP88.
(Peptide available as
This antibody gave a positive signal in HeLa whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
Essential component of nuclear pore complex.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nuclear pore complex.
Information by UniProt
88 kDa nucleoporin antibody
Nuclear pore complex protein 88 antibody
Western blot - Anti-NUP88 antibody (ab79785)
Anti-NUP88 antibody (ab79785) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
87 kDa (
why is the actual band size different from the predicted?
Nuclear pore complex protein Nup88
contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (87 kDa) than predicted (83 kDa).
Immunocytochemistry/ Immunofluorescence - Anti-NUP88 antibody (ab79785) Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab79785 (1/200) staining NUP88 in HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highligh the nucleus (red). Please refer to Abreview for further experimental details.
has not yet been referenced specifically in any publications.
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