Anti-NUP43 抗体 (ab69447)
Key features and details
- Mouse polyclonal to NUP43
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NUP43 antibody -
製品の詳細
Mouse polyclonal to NUP43 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human NUP43. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human placenta tissue lysate; NUP43 transfected 293T lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69447の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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関連性
NUP43 probably mediates the assembly of subdomains of the nuclear pore complex (NPC) or facilitates the interaction of transport complexes with the NPC. -
細胞内局在
Nucleus; nuclear pore complex. -
参照データベース
- Entrez Gene: 348995 Human
- Omim: 608141 Human
- SwissProt: Q8NFH3 Human
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別名
- bA350J20.1 antibody
- FLJ13287 antibody
- Nucleoporin 43kDa antibody
see all
画像
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Anti-NUP43 antibody (ab69447) at 1/500 dilution + human placenta tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-NUP43 antibody (ab69447) at 1/500 dilution
Lane 1 : NUP43 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
ab69447 at 10µg/ml staining NUP43 in Hela cells by ICC/IF.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab69447 は論文での使用が確認できていません。