The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 19339552
1/50 - 1/250.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa).
Use at 2-5 µg/mg of lysate.
May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
Note=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene. Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET.
Contains FG repeats.
Probably glycosylated as it reacts with wheat germ agglutinin (WGA).
Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling NUP214 with ab70497 at 1/100 (2µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Western blot - Anti-NUP214 antibody (ab70497)
All lanes : Anti-NUP214 antibody (ab70497) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of Human NUP214 by Western Blot of Immunprecipitate.
ab70497 at 1µg/ml staining NUP214 in HeLa whole cell lysates immunoprecipitated using ab70497 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Chemiluminescence with exposure time of 10 seconds.
Port SA et al. The Oncogenic Fusion Proteins SET-Nup214 and Sequestosome-1 (SQSTM1)-Nup214 Form Dynamic Nuclear Bodies and Differentially Affect Nuclear Protein and Poly(A)+ RNA Export. J Biol Chem291:23068-23083 (2016).
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