All lanes : Anti-NUP188 antibody (ab86601) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 196 kDa Observed band size: 196 kDa
Exposure time: 3 minutes
Immunoprecipitation - NUP188 antibody (ab86601)
Detection of NUP188 by Western Blot of Immunprecipitate.
ab86601 at 1µg/ml staining NUP188 in HeLa whole cell lysate immunoprecipitated using ab86601 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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