All lanes : Anti-NUP160 antibody (ab74147) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells Lane 2 : Extracts from NIH/3T3 cells Lane 3 : Extracts from A549 cells Lane 4 : Extracts from NIH/3T3 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 149 kDa Observed band size : 149 kDa
Immunocytochemistry/ Immunofluorescence - NUP160 antibody (ab74147)Image courtesy of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canada
ab74147 (1/100) staining NUP160 in human HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). Secondary antibody was goat anti-rabbit IgG conjugated to FITC. For further experimental details please refer to Abreview.
Yokoyama H et al. The nucleoporin MEL-28 promotes RanGTP-dependent ?-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun5:3270 (2014).
Read more (PubMed: 24509916) »
Tarazón E et al. Heart failure induces significant changes in nuclear pore complex of human cardiomyocytes. PLoS One7:e48957 (2012).
Read more (PubMed: 23152829) »