製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 154 kDa (predicted molecular weight: 154 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 23423481

ターゲット情報

  • 機能Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
  • 配列類似性Contains 4 RanBP2-type zinc fingers.
  • ドメインContains F-X-F-G repeats.
  • 細胞内局在Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 153 kDa nucleoporin antibody
    • HNUP153 antibody
    • N153 antibody
    • NU153_HUMAN antibody
    • Nuclear pore complex protein hnup153 antibody
    • Nuclear pore complex protein Nup153 antibody
    • Nucleoporin 153kDa antibody
    • Nucleoporin Nup153 antibody
    • Nup 153 antibody
    • Nup153 antibody
    see all

Anti-Nup153 antibody [QE5] 画像

  • ICC/IF image of ab24700 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24700, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Methanol fixed HeLa stained with ab24700. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with Giantin (yellow).
  • Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 4% formaldehyde and permeabilized in 0.2% Triton x100 for 10 minutes at room temperature, then washed 3 times in PBS. The fixation and permeabilization was carried out in a single step. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).

    A: HeLa cells, ab24700 used at 4µg/ml
    B: 3T3 cells, ab24700 used at 2µg/ml
    C: 3T3 cells, ab24700 used at 4µg/ml
    D: 3T3 cells, ab24700 used at 6.6µg/ml

  • Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 100% methanol for 20 minutes at -20°C, then washed once in PBS. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).

    A: HeLa cells, ab24700 used at 4µg/ml
    B: 3T3 cells, ab24700 used at 2µg/ml
    C: 3T3 cells, ab24700 used at 4µg/ml
    D: 3T3 cells, ab24700 used at 6.6µg/ml

  • Overlay histogram showing HeLa cells stained with ab24700 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24700, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-Nup153 antibody [QE5] (ab24700) at 1/1000 dilution + Human U2OS nuclear lysate at 40 µg

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 154 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Nup153 antibody [QE5] (ab24700) 使用論文

This product has been referenced in:
  • Ilgen P  et al. RESOLFT Nanoscopy of Fixed Cells Using a Z-Domain Based Fusion Protein for Labelling. PLoS One 10:e0136233 (2015). Read more (PubMed: 26375606) »
  • Choudhary RK  et al. Comparison of the transcriptomes of long-term label retaining-cells and control cells microdissected from mammary epithelium: an initial study to characterize potential stem/progenitor cells. Front Oncol 3:21 (2013). IHC-P ; Cow . Read more (PubMed: 23423481) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Permeabilization Yes - 0.5% Triton
Specification U2OS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 27 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Permeabilization Yes - NP40
Specification U2OS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 15 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - nuclear (U2OS)
Specification U2OS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Apr 22 2014

Thank you for your enquiry. The antibody was raised against rat liver nuclear envelope proteins extracted in TX buffer (50 mM triethanolamine [TEA], pH 7.4, 500 mM NaCI, 0.5 % Triton X-100, 1 mM DTT, 1 mM PMSF and 1:1,000 CLAP [10 mg/ml each of chy...

Read More

Thank you for your enquiry. I have been in touch with the source of this antibody and unfortunately I do not have a western image available at this time. I do however have soem images used to QC ths image by immunofluorescence. I have sent a follow ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"