Anti-Nup107 抗体 (ab85916)
Key features and details
- Rabbit polyclonal to Nup107
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Nup107 antibody
Nup107 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nup107 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between residues 875 and 925 of Human Nup107 (NP_065134.1)
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab85916 was affinity purified using an epitope specific to Nup107 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85916の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. -
組織特異性
Ubiquitously expressed in fetal and adult tissues. -
関連疾患
Nephrotic syndrome 11 -
配列類似性
Belongs to the nucleoporin Nup84/Nup107 family. -
細胞内局在
Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755). - Information by UniProt
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参照データベース
- Entrez Gene: 57122 Human
- Omim: 607617 Human
- SwissProt: P57740 Human
- Unigene: 524574 Human
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別名
- 107 kDa nucleoporin antibody
- NU107_HUMAN antibody
- Nuclear pore complex protein Nup107 antibody
see all
画像
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All lanes : Anti-Nup107 antibody (ab85916) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutes -
Detection of Nup107 by Western Blot of Immunprecipitate.
ab85916 at 1µg/ml staining Nup107 in HeLa whole cell lysate immunoprecipitated using ab85916 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab85916 は 2 報の論文で使用されています。
- Yokoyama H et al. The nucleoporin MEL-28 promotes RanGTP-dependent ?-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun 5:3270 (2014). PubMed: 24509916
- Light WH et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). PubMed: 23555195