The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
Use at 2-5 µg/mg of lysate.
機能Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
配列類似性Belongs to the nucleoporin Nup84/Nup107 family.
細胞内局在Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
All lanes : Anti-Nup107 antibody (ab85916) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 106 kDa Observed band size : 106 kDa
Exposure time : 3 minutes
Immunoprecipitation - Nup107 antibody (ab85916)
Detection of Nup107 by Western Blot of Immunprecipitate.
ab85916 at 1µg/ml staining Nup107 in HeLa whole cell lysate immunoprecipitated using ab85916 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-Nup107 antibody (ab85916) 使用論文
This product has been referenced in:
Yokoyama H et al. The nucleoporin MEL-28 promotes RanGTP-dependent ?-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun5:3270 (2014).
Read more (PubMed: 24509916) »
Light WH et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol11:e1001524 (2013).
Read more (PubMed: 23555195) »