The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
Use at 2-5 µg/mg of lysate.
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
Ubiquitously expressed in fetal and adult tissues.
Nephrotic syndrome 11
Belongs to the nucleoporin Nup84/Nup107 family.
Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
All lanes : Anti-Nup107 antibody (ab85916) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 106 kDa Observed band size: 106 kDa
Exposure time: 3 minutes
Immunoprecipitation - Nup107 antibody (ab85916)
Detection of Nup107 by Western Blot of Immunprecipitate.
ab85916 at 1µg/ml staining Nup107 in HeLa whole cell lysate immunoprecipitated using ab85916 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Yokoyama H et al. The nucleoporin MEL-28 promotes RanGTP-dependent ?-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun5:3270 (2014).
Read more (PubMed: 24509916) »
Light WH et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol11:e1001524 (2013).
Read more (PubMed: 23555195) »