製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nup107
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 875 and 925 of Human Nup107 (NP_065134.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
  • 組織特異性
    Ubiquitously expressed in fetal and adult tissues.
  • 関連疾患
    Nephrotic syndrome 11
  • 配列類似性
    Belongs to the nucleoporin Nup84/Nup107 family.
  • 細胞内局在
    Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
  • Information by UniProt
  • 参照データベース
  • 別名
    • 107 kDa nucleoporin antibody
    • NU107_HUMAN antibody
    • Nuclear pore complex protein Nup107 antibody
    • Nucleoporin 107kDa antibody
    • Nucleoporin Nup107 antibody
    • NUP107 antibody
    • NUP84 antibody
    • NUP84, yeast, homolog of antibody
    • OTTHUMP00000240604 antibody
    • OTTHUMP00000240606 antibody
    see all

画像

  • All lanes : Anti-Nup107 antibody (ab85916) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 106 kDa
    Observed band size : 106 kDa


    Exposure time : 3 minutes
  • Detection of Nup107 by Western Blot of Immunprecipitate.
    ab85916 at 1µg/ml staining Nup107 in HeLa whole cell lysate immunoprecipitated using ab85916 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

This product has been referenced in:
  • Yokoyama H  et al. The nucleoporin MEL-28 promotes RanGTP-dependent ?-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun 5:3270 (2014). Read more (PubMed: 24509916) »
  • Light WH  et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). Read more (PubMed: 23555195) »

See all 2 Publications for this product

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