製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5% Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Tested in peptide ELISA: antibody detection limit dilution >1/32000.
    Western Blot: Use at an assay dependent dilution.
    We would welcome any customer feedback about ab5641.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.
    • 配列類似性
      Contains 1 PID domain.
    • ドメイン
      The PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B.
    • 細胞内局在
      Cytoplasm. Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CAG 3A antibody
      • CAG3A antibody
      • CTG 3a antibody
      • CTG3a antibody
      • NBL antibody
      • NUMB Drosophilia Homolog Like antibody
      • Numb homolog (Drosophila) like antibody
      • Numb homolog like antibody
      • Numb like protein antibody
      • NUMB R antibody
      • Numb-like protein antibody
      • Numb-R antibody
      • Numb-related gene antibody
      • Numb-related protein antibody
      • NUMBL antibody
      • NUMBL_HUMAN antibody
      • NUMBLIKE antibody
      • NUMBR antibody
      • TNRC 23 antibody
      • TNRC23 antibody
      • Trinucleotide repeat-containing gene 23 antibody
      see all

    画像

    • Lanes 1 - 2 : Anti-NUMBL antibody (ab5641) at 1 µg/ml
      Lane 3 : anti-MYC Tag at 1/1000 dilution

      Lane 1 : HEK293 Lysate overexpressing Human Numbl with C-terminal MYC tag
      Lane 2 : Mock transfected HEK293
      Lane 3 : HEK293 Lysate overexpressing Human Numbl with C-terminal MYC tag

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique

      Primary incubations were for 1 hour

    プロトコール

    参考文献

    This product has been referenced in:
    • Tokumitsu H  et al. Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinases. J Biol Chem 280:35108-18 (2005). Read more (PubMed: 16105844) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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