製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NUMBL
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human NUMBL.

    (Peptide available as ab37499.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Brain (Mouse) Whole Cell Lysate Testis (Mouse) Whole Cell Lysate Pancreas (Mouse) Whole Cell Lysate Lung (Mouse) Whole Cell Lysate Brain (Mouse) Tissue Lysate Spinal Cord (Mouse) Tissue Lysate Brain (Rat) Tissue Lysate - normal tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 20079715
IHC-FoFr 1/1000.

ターゲット情報

  • 機能
    Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.
  • 配列類似性
    Contains 1 PID domain.
  • ドメイン
    The PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B.
  • 細胞内局在
    Cytoplasm. Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAG 3A antibody
    • CAG3A antibody
    • CTG 3a antibody
    • CTG3a antibody
    • NBL antibody
    • NUMB Drosophilia Homolog Like antibody
    • Numb homolog (Drosophila) like antibody
    • Numb homolog like antibody
    • Numb like protein antibody
    • NUMB R antibody
    • Numb-like protein antibody
    • Numb-R antibody
    • Numb-related gene antibody
    • Numb-related protein antibody
    • NUMBL antibody
    • NUMBL_HUMAN antibody
    • NUMBLIKE antibody
    • NUMBR antibody
    • TNRC 23 antibody
    • TNRC23 antibody
    • Trinucleotide repeat-containing gene 23 antibody
    see all

画像

  • All lanes : Anti-NUMBL antibody (ab37500) at 1 µg/ml

    Lane 1 : Brain (Mouse) Whole Cell Lysate - normal tissue
    Lane 2 : Testis (Mouse) Whole Cell Lysate - normal tissue
    Lane 3 : Mouse pancreas tissue lysate - total protein (ab29363)
    Lane 4 : Lung (Mouse) Whole Cell Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-NUMBL antibody (ab37500) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 65-70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab37500 staining NUMBL in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

  • ICC/IF image of ab37500 stained pc12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37500, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Tao T  et al. Numbl inhibits glioma cell migration and invasion by suppressing TRAF5-mediated NF-?B activation. Mol Biol Cell 23:2635-44 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22593207) »
  • Liu C  et al. Epigenetic regulation of miR-184 by MBD1 governs neural stem cell proliferation and differentiation. Cell Stem Cell 6:433-44 (2010). WB ; Mouse . Read more (PubMed: 20452318) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (retina, brain)
Specification
retina, brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Dr. Ryan Macdonald

Verified customer

投稿 Nov 17 2014

ab37500 is unlikely to recognise NUMB since the sequence alignment shows only 35% homology between NUMB and the immunogen of ab37500.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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