Anti-NUMB 抗体 [1A7] (ab123891)


  • 製品名Anti-NUMB antibody [1A7]
    NUMB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1A7] to NUMB
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human recombinant NUMB produced in HEK293T cells (NP_003735).

  • ポジティブ・コントロール
    • COS7 cells transiently transfected by pCMV6-ENTRY NUMB. Lysate from HEK293 cells transiently transfected by pCMV6-ENTRY NUMB.



Our Abpromise guarantee covers the use of ab123891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 71 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.


  • 機能Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
  • 配列類似性Contains 1 PID domain.
  • 翻訳後修飾Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c14_5527 antibody
    • C14orf41 antibody
    • FLJ31314 antibody
    • h Numb antibody
    • h-Numb antibody
    • Numb antibody
    • Numb homolog (Drosophila) antibody
    • Numb homolog antibody
    • Numb protein homolog antibody
    • NUMB_HUMAN antibody
    • Protein numb homolog antibody
    • Protein S171 antibody
    • S171 antibody
    see all

Anti-NUMB antibody [1A7] 画像

  • All lanes : Anti-NUMB antibody [1A7] (ab123891) at 1/2000 dilution

    Lane 1 : lysate from HEK293T cells transfected with the pCMV6-ENTRY control cDNA
    Lane 2 : lysate from HEK293T cells transfected with the pCMV6-ENTRY NUMB cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216230)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB using 1/100 ab123891.
  • HEK293T cells transfected with either NUMB overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab123891 at 1/100 dilution, and then analyzed by flow cytometry.

Anti-NUMB antibody [1A7] (ab123891) 使用論文

ab123891 has not yet been referenced specifically in any publications.

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Unfortunately the exact epitope sight for ab123891 has not been mapped however based on the IF protocol that was used to test the antibody, we believe that the epitope may be intracellular (as they had to use 0.1% T...

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