製品の概要

  • 製品名
    Anti-NuMA antibody
    NuMA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NuMA
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 1 and 309 of Human NuMA (AAH04165).

  • ポジティブ･コントロール
    • 293T, A431, H1299, HeLa, MOLT4 or Raji cell lines.

法規制情報

医薬用外毒物

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 238 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    May be a structural component of the nucleus.
  • 細胞内局在
    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
      • NMP 22 antibody
      • Nuclear matrix protein 22 antibody
      • Nuclear mitotic apparatus protein 1 antibody
      • Nuclear mitotic apparatus protein antibody
      • NUMA 1 antibody
      • NUMA antibody
      • NuMA protein antibody
      • NUMA1 antibody
      • NUMA1_HUMAN antibody
      • SP H antigen antibody
      • SP-H antigen antibody
      • Structural nuclear protein antibody
      see all

    画像

    • Western blot - Anti-NuMA antibody (ab97585)
      Western blot - Anti-NuMA antibody (ab97585)
      Anti-NuMA antibody (ab97585) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 238 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody (ab97585)
      Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody (ab97585)
      ab97585, at 1/200, staining paraformaldehyde-fixed A431 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab97585)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab97585)
      ab97585, at 1/100, staining paraffin-embedded Cal27 Xenograft by Immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Millar MW  et al. The Adhesion G-Protein-Coupled Receptor, GPR56/ADGRG1, Inhibits Cell-Extracellular Matrix Signaling to Prevent Metastatic Melanoma Growth. Front Oncol 8:8 (2018). Read more (PubMed: 29450192) »
    • Zorniak M  et al. Yeast display biopanning identifies human antibodies targeting glioblastoma stem-like cells. Sci Rep 7:15840 (2017). Read more (PubMed: 29158489) »

    See all 7 Publications for this product

    ab97585 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NuMA antibody

     Poor
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Sample
    Human Tissue sections (Engineerd construct)
    Specification
    Engineerd construct
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    Username

    Mr. Hendrik Gremmels

    Verified customer

    投稿 Jan 16 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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