Anti-NuMA 抗体 (ab84680)
Key features and details
- Rabbit polyclonal to NuMA
- Suitable for: IHC-P
- Reacts with: Human, Common marmoset
- Isotype: IgG
製品の概要
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製品名
Anti-NuMA antibody
NuMA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NuMA -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human, Common marmoset
交差が予測される動物種: Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residue 900 and 950 of Human NuMA (NP_006176.2).
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ポジティブ・コントロール
- IHC-P: Human breast carcinoma, seminoma and ovarian tumor tissue. Common marmoset testis tissue.
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特記事項
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84680の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/100 - 1/500.
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特記事項 |
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IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
May be a structural component of the nucleus. -
細胞内局在
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. - Information by UniProt
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参照データベース
- Entrez Gene: 4926 Human
- Omim: 164009 Human
- SwissProt: Q14980 Human
- Unigene: 325978 Human
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別名
- Centrophilin stabilizes mitotic spindle in mitotic cells antibody
- NMP 22 antibody
- Nuclear matrix protein 22 antibody
see all
画像
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ab84680 staining NuMA in Marmoset (common) testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Buoin's fixative, permeabilized in Triton X-100 (1/100 in PBS) and blocked with 25% serum for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 17 hours at 4°C. A Biotin-conjugated Chicken anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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ab84680, at 1/100 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
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ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using DAB staining.
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ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB staining.
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ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human ovarian tumor by Immunohistochemistry using DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab84680 は 10 報の論文で使用されています。
- Liu X et al. 3D heterospecies spheroids of pancreatic stroma and cancer cells demonstrate key phenotypes of pancreatic ductal adenocarcinoma. Transl Oncol 14:101107 (2021). PubMed: 33946033
- Bayerl J et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28:1549-1565.e12 (2021). PubMed: 33915080
- Petrus-Reurer S et al. Preclinical safety studies of human embryonic stem cell-derived retinal pigment epithelial cells for the treatment of age-related macular degeneration. Stem Cells Transl Med 9:936-953 (2020). PubMed: 32319201
- Dimitracopoulos A et al. Mechanochemical Crosstalk Produces Cell-Intrinsic Patterning of the Cortex to Orient the Mitotic Spindle. Curr Biol 30:3687-3696.e4 (2020). PubMed: 32735816
- Bendriem RM et al. Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. Elife 8:N/A (2019). PubMed: 31794381
- Lu R et al. Neurons generated from carcinoma stem cells support cancer progression. Signal Transduct Target Ther 2:16036 (2017). PubMed: 29263908
- Huang CW et al. The Differentiation Stage of Transplanted Stem Cells Modulates Nerve Regeneration. Sci Rep 7:17401 (2017). PubMed: 29234013
- Shangguan W et al. Endothelium originated from colorectal cancer stem cells constitute cancer blood vessels. Cancer Sci 108:1357-1367 (2017). IHC-Fr . PubMed: 28421697
- Petrus-Reurer S et al. Integration of Subretinal Suspension Transplants of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells in a Large-Eyed Model of Geographic Atrophy. Invest Ophthalmol Vis Sci 58:1314-1322 (2017). PubMed: 28241319
- Sugawa F et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J 34:1009-24 (2015). IHC-P ; Human . PubMed: 25750208