製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NuMA
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Fr, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Chicken, Human
    交差が予測される動物種: Mammal
  • 免疫原

    Recombinant full length protein corresponding to Human NuMA.

  • ポジティブ・コントロール
    • HeLa whole cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.
WB 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 238 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP 1/10 - 1/500.

ターゲット情報

  • 機能
    May be a structural component of the nucleus.
  • 細胞内局在
    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody 画像

  • ab36999 at 1/1000 dilution, staining human HeLa cells fixed with 3.5% formaldehyde, by immunofluorescence, showing the typical pattern for NuMa in the cell nucleus during interphase and on spindles during mitosis.

Anti-NuMA antibody (ab36999) 使用論文

This product has been referenced in:
  • Platani M  et al. Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes. J Cell Biol 210:45-62 (2015). ICC/IF ; Human . Read more (PubMed: 26124292) »
  • Kulukian A  et al. Epidermal development, growth control, and homeostasis in the face of centrosome amplification. Proc Natl Acad Sci U S A 112:E6311-20 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26578791) »

See all 13 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK cells)
Specification
MDCK cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Username

Dr. Inma Banon

Verified customer

投稿 Sep 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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