製品の概要

  • 製品名Anti-NuMA antibody [A73-B/D12]
    NuMA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [A73-B/D12] to NuMA
  • 特異性Reacts with an intracellular 228 kD protein found in the nucleus during interphase.
  • アプリケーション適用あり: IP, IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Live Ls 174T cells (human colon carcinoma).

  • ポジティブ・コントロール
    • Tonsil, Thymus, Spleen.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファーpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名A73-B/D12
  • アイソタイプIgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/100 - 1/300. Detects a band of approximately 240 kDa.

ターゲット情報

  • 機能May be a structural component of the nucleus.
  • 細胞内局在Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody [A73-B/D12] (ab5675) 使用論文

This product has been referenced in:
  • Shi JG  et al. Tissue engineering of ureteral grafts by seeding urothelial differentiated hADSCs onto biodegradable ureteral scaffolds. J Biomed Mater Res A 100:2612-22 (2012). ICC/IF . Read more (PubMed: 22615210) »
  • Kwong J  et al. An antagonist of the chemokine receptor CXCR4 induces mitotic catastrophe in ovarian cancer cells. Mol Cancer Ther 8:1893-905 (2009). Read more (PubMed: 19567818) »

See all 2 Publications for this product

Product Wall

Thanks for your enquiry.

Regarding the immunogen information:
ab5675 immunogen is Live Ls 174T cells and epitope is not known.

RE: could you provide Ab86129 and/or Ab84680 without BSA?
These 2 antibodies are not typically ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Ovarian carcinoma)
Specification Ovarian carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 16 2009

Thank you for your e-mail. This is to let you know that we have placed a new order for you one vial of ab5675 as a replacement vial from a new batch. For your information, the new order number is 80286. We hope this new vial will work. Please do l...

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Thank you for your reply. We are very sorry that you are not satisfied with our response. This antibody has been tested and characterized for IHC using human colon carcinoma. Unfortunately, we have no information if this product reacts with HeLa ce...

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Thank you for your enquiry. We are very sorry to hear that you are having problem with this antibody. We have looked at our computer system which stores all the technical enquiries, complaints and correspondence. Unfortunately, we have not received...

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Thank you for your enquiry. We unfortunately do not have an image available for ab5675. The antibody has been tested for application in Western blot, Immunohistochemistry (Formalin-fixed paraffin-embedded sections), and Immunohistochemistry (Frozen sec...

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Thank you so much for your patience. I had to contact the originator of ab5675 and have just heard back. Concerning ab5675, the antibody is red in color because it is concentrated medium from cell culture containing phenol red. The other antibody, ab30...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"