The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 40 kDa for the recombinant protein fragment (predicted molecular weight for the full length protein: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
NUFIP1 contains 1 C2H2- type zinc finger and binds RNA. It interacts with FMR1. NUFIP1 may be associated with preinitiation complexes, open transcription complexes, and elongation complexes. It may facilitate ATP-dependent dissociation of hyperphosphorylated pol II from open transcription complexes.