Rabbit polyclonal to Nuf2
The Immunogen used was a recombinant protein corresponding to human CDCA1
WB: 293T, A431, H1299, MOLT4 or Raji cell lysate;
ICC/IF: A431 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
1/100 - 1/200.
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Belongs to the NUF2 family.
Can be phosphorylated by AURKA and AURKB.
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Information by UniProt
Cancer/testis antigen 106 antibody
CDCA 1 antibody
Western blot - Anti-Nuf2 antibody (ab96147)
Anti-Nuf2 antibody (ab96147) at 1/3000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 54 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Nuf2 antibody (ab96147)
Top panel: ab96147, at a 1/200 dilution, staining Nuf2 in paraformaldehyde-fixed A431 cell by immunofluorescence.
Lower panel: merged with DNA probe.
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