製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nuf2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The Immunogen used was a recombinant protein corresponding to human CDCA1

  • ポジティブ・コントロール
    • WB: 293T, A431, H1299, MOLT4 or Raji cell lysate; ICC/IF: A431 cells.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • 配列類似性
    Belongs to the NUF2 family.
  • 翻訳後修飾
    Can be phosphorylated by AURKA and AURKB.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 106 antibody
    • CDCA 1 antibody
    • CDCA1 antibody
    • Cell division cycle associated 1 antibody
    • Cell division cycle associated protein 1 antibody
    • Cell division cycle-associated protein 1 antibody
    • CT106 antibody
    • hNuf 2 antibody
    • hNuf2 antibody
    • hNuf2R antibody
    • hsNuf2 antibody
    • Kinetochore protein Nuf2 antibody
    • Nuf 2 antibody
    • NUF2 antibody
    • NUF2 NDC80 kinetochore complex component antibody
    • NUF2 NDC80 kinetochore complex component homolog antibody
    • NUF2, S. cerevisiae, homolog of antibody
    • NUF2_HUMAN antibody
    • NUF2R antibody
    see all

Anti-Nuf2 antibody 画像

  • Anti-Nuf2 antibody (ab96147) at 1/3000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 54 kDa
  • Top panel: ab96147, at a 1/200 dilution, staining Nuf2 in paraformaldehyde-fixed A431 cell by immunofluorescence.
    Lower panel: merged with DNA probe.

Anti-Nuf2 antibody (ab96147) 使用論文

ab96147 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab96147.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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