製品の概要

  • 製品名Anti-Nuf2 antibody
    Nuf2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nuf2
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Chicken, Human
    交差が予測される動物種: Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide:

    CGGDSYAKIDEKTAELKRKMFKMS

    , corresponding to C terminal amino acids 443-463 of Human Nuf2.

  • ポジティブ・コントロール
    • HeLa cells and cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab122962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ICC/IF 1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining.

ターゲット情報

  • 機能Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • 配列類似性Belongs to the NUF2 family.
  • 翻訳後修飾Can be phosphorylated by AURKA and AURKB.
  • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 106 antibody
    • CDCA 1 antibody
    • CDCA1 antibody
    • Cell division cycle associated 1 antibody
    • Cell division cycle associated protein 1 antibody
    • Cell division cycle-associated protein 1 antibody
    • CT106 antibody
    • hNuf 2 antibody
    • hNuf2 antibody
    • hNuf2R antibody
    • hsNuf2 antibody
    • Kinetochore protein Nuf2 antibody
    • Nuf 2 antibody
    • NUF2 antibody
    • NUF2 NDC80 kinetochore complex component antibody
    • NUF2 NDC80 kinetochore complex component homolog antibody
    • NUF2, S. cerevisiae, homolog of antibody
    • NUF2_HUMAN antibody
    • NUF2R antibody
    see all

Anti-Nuf2 antibody 画像

  • Anti-Nuf2 antibody (ab122962) at 1/5000 dilution + HeLa cell lysate

    Predicted band size : 54 kDa
  • ab122962 at 1/500 dilution staining Nuf2 in Methanol-fixed HeLa cells by Immunofluorescence (green) showing localization to kinetochores at the stages of prometaphase to anaphase. Chromosomes were stained by DAPI (violet).

Anti-Nuf2 antibody (ab122962) 使用論文

This product has been referenced in:
  • Hu P  et al. siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Biosci Rep 35:N/A (2015). WB, IHC-P ; Human . Read more (PubMed: 25370920) »
  • Xu P  et al. B56-PP2A regulates motor dynamics for mitotic chromosome alignment. J Cell Sci 127:4567-73 (2014). WB ; Human . Read more (PubMed: 25179604) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Permeabilization No
Specification Colon carcinoma
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Feb 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"