Anti-Nuf2 抗体 (ab122962)
Key features and details
- Rabbit polyclonal to Nuf2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Nuf2 antibody
Nuf2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nuf2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rhesus monkey, Gorilla -
免疫原
Synthetic peptide:
CGGDSYAKIDEKTAELKRKMFKMS
, corresponding to C terminal amino acids 443-463 of Human Nuf2. -
ポジティブ・コントロール
- HeLa cells and cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122962の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
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ICC/IF | (1) |
1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining.
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特記事項 |
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WB
1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
ICC/IF
1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining. |
ターゲット情報
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機能
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
配列類似性
Belongs to the NUF2 family. -
翻訳後修飾
Can be phosphorylated by AURKA and AURKB. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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参照データベース
- Entrez Gene: 101139481 Gorilla
- Entrez Gene: 83540 Human
- Entrez Gene: 693637 Rhesus monkey
- Omim: 611772 Human
- SwissProt: Q9BZD4 Human
- Unigene: 651950 Human
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別名
- Cancer/testis antigen 106 antibody
- CDCA 1 antibody
- CDCA1 antibody
see all
画像
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Anti-Nuf2 antibody (ab122962) at 1/5000 dilution + HeLa cell lysate
Predicted band size: 54 kDa -
ab122962 at 1/500 dilution staining Nuf2 in Methanol-fixed HeLa cells by Immunofluorescence (green) showing localization to kinetochores at the stages of prometaphase to anaphase. Chromosomes were stained by DAPI (violet).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab122962 は 8 報の論文で使用されています。
- Zhu X et al. ANP32E contributes to gastric cancer progression via NUF2 upregulation. Mol Med Rep 26:N/A (2022). PubMed: 35795988
- Papini D et al. The Aurora B gradient sustains kinetochore stability in anaphase. Cell Rep 37:109818 (2021). PubMed: 34758321
- Zhang N et al. Osteopontin accelerates the development and metastasis of bladder cancer via activating JAK1/STAT1 pathway. Genes Genomics 42:467-475 (2020). PubMed: 32088853
- Nourreddine S et al. NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay. Cell Rep 31:107660 (2020). PubMed: 32433969
- Addis Jones O et al. PLK1 facilitates chromosome biorientation by suppressing centromere disintegration driven by BLM-mediated unwinding and spindle pulling. Nat Commun 10:2861 (2019). PubMed: 31253795
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). PubMed: 29968190
- Hu P et al. siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Biosci Rep 35:N/A (2015). WB, IHC-P ; Human . PubMed: 25370920
- Xu P et al. B56-PP2A regulates motor dynamics for mitotic chromosome alignment. J Cell Sci 127:4567-73 (2014). WB ; Human . PubMed: 25179604