製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NUDT18
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 252-301 (KGLLGLQHLG RDHSDGICLN VLVTVAFRSP GIQDEPPKVR GENFSWWKVM) of Human NUDT18 (NP_079091).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Probably mediates the hydrolysis of some nucleoside diphosphate derivatives.
  • 配列類似性
    Belongs to the Nudix hydrolase family.
    Contains 1 nudix hydrolase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2-hydroxy-dADP phosphatase antibody
    • 7,8-dihydro-8-oxoguanine phosphatase antibody
    • 8-oxo-dGDP phosphatase NUDT18 antibody
    • BC036718 antibody
    • FLJ22494 antibody
    • MGC38179 antibody
    • MTH3 antibody
    • mutT homolog 3 antibody
    • mutT human homolog 3 antibody
    • Nucleoside diphosphate linked moiety X motif 18 antibody
    • Nucleoside diphosphate-linked moiety X motif 18 antibody
    • NUD18_HUMAN antibody
    • Nudix (nucleoside diphosphate linked moiety X) type motif 18 antibody
    • Nudix motif 18 antibody
    • NUDT 18 antibody
    • Nudt18 antibody
    • RGD1311802 antibody
    see all

画像

  • Anti-NUDT18 antibody (ab82997) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

プロトコール

参考文献

ab82997 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293T cells transfected)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
HEK293T cells transfected
Blocking step
Blocking Ace as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

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投稿 Apr 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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