製品の概要

  • 製品名Anti-Nucleostemin antibody
    Nucleostemin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nucleostemin
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
    交差が予測される動物種: Rabbit, Horse, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region between residue 500 and the C-terminus (residue 549) of human Nucleostemin (NP_055181.3)

  • ポジティブ・コントロール
    • HeLa, 293T and mouse 3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 機能May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
  • 組織特異性Increased levels in lung tissue in cancer patients.
  • 配列類似性Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • ドメインThe basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • 細胞内局在Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C77032 antibody
    • E2 induced gene 3 protein antibody
    • E2-induced gene 3 protein antibody
    • E2IG3 antibody
    • Estradiol induced nucleotide binding protein antibody
    • GNL3 antibody
    • GNL3_HUMAN antibody
    • Guanine nucleotide binding protein like 3 (nucleolar) antibody
    • Guanine nucleotide binding protein like 3 antibody
    • Guanine nucleotide-binding protein-like 3 antibody
    • MGC800 antibody
    • NNP47 antibody
    • Novel nucleolar protein 47 antibody
    • NS antibody
    • Nucleolar GTP binding protein 3 antibody
    • Nucleolar GTP-binding protein 3 antibody
    • Nucleostemin antibody
    • Putative nucleotide binding protein estradiol induced antibody
    see all

Anti-Nucleostemin antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling Nucleostemin with ab70346 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-Nucleostemin antibody (ab70346) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : mouse 3T3 whole cell lysate at 50 µg


    Predicted band size : 62 kDa
    Observed band size : 62 kDa


    Exposure time : 10 seconds
  • Detection of Nucleostemin by Western Blot of Immunprecipitate. All lanes: ab70346 at 1µg/ml staining Nucleostemin in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies: Lane 1: an irrelevant antibody. Lane 2: ab70345 Lane 3: ab70346. Detection: Chemiluminescence with exposure time of 3 seconds
  • Immunohistochemistical detection of Nucleostemin with ab70346 on formaldehyde-fixed paraffin-embedded human colon sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Blocking step: 1% BSA  for 10 mins @ rt°C. Primary antibody ab70346 incubated at 1/50 for 16 hours in TBS/BSA/azide. Secondary antibody: anti-rabbit IgG conjugated to biotin (1/200).  The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. Stem cell markers have been observed in cycling columnar cells at the crypt base (Nature 449, 1003-1007; 2007). The cells (nuclei of the simple columnar epithelium of the upper mucosa) immunoreactive for Nucleostemin in this image (green arrowheads) anatomically correspond to the same cells expected to be Lgr5 positivite. A red arrowhead indicates nucleolar positivity in one of many cell nuclei in the Lamina propria of the mucosa (this area is not known for stem cell posit

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  • ICC/IF image of ab70346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70346, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Nucleostemin antibody (ab70346) 使用論文

This product has been referenced in:
  • Allen KD  et al. Nucleolar integrity is required for the maintenance of long-term synaptic plasticity. PLoS One 9:e104364 (2014). ICC/IF ; Mouse . Read more (PubMed: 25089620) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10 % GEL, SDS)
Sample Human Cell lysate - whole cell (HEK293, HELA, HCT116)
Specification HEK293, HELA, HCT116
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

投稿 Apr 24 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Human Cell (HEK293)
Specification HEK293
Permeabilization Yes - TRITON
Fixative Methanol
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投稿 Apr 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela Cells)
Specification Hela Cells
Fixative Paraformaldehyde
Permeabilization Yes - .25% Triton X-100 in PBS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Ms. Western,IP ChIP

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投稿 Oct 22 2011

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Application Western blot
Sample Human Tissue lysate - whole (Hela Cells)
Loading amount 30 µg
Specification Hela Cells
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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投稿 Oct 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse ES cells)
Loading amount 30 µg
Specification Mouse ES cells
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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投稿 Oct 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela Cells)
Total protein in input 200000 µg
Specification Hela Cells
Immuno-precipitation step Protein G
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投稿 Oct 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
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Mr. Carl Hobbs

Verified customer

投稿 Feb 15 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (chick day 6 embryo liver and kidney)
Specification chick day 6 embryo liver and kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 15 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (adult rat brain)
Specification adult rat brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"