Anti-Nucleoside phosphorylase 抗体 [EPR5715] (ab109447)


  • 製品名Anti-Nucleoside phosphorylase antibody [EPR5715]
    Nucleoside phosphorylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Nucleoside Phosphorylase.

  • ポジティブ・コントロール
    • Jurkat, JAR, K562, Human placenta, and 293T lysates; SH SY5Y cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値) KD = 1.76 x 10 -10 M Learn more about KD
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR5715
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab109447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 関連疾患Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.
    • 配列類似性Belongs to the PNP/MTAP phosphorylase family.
    • 細胞内局在Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ94043 antibody
      • FLJ97288 antibody
      • FLJ97312 antibody
      • Inosine phosphorylase antibody
      • Inosine-guanosine phosphorylase antibody
      • MGC117396 antibody
      • MGC125915 antibody
      • MGC125916 antibody
      • NP antibody
      • Np1 antibody
      • Nucleoside phosphorylase antibody
      • PNP antibody
      • Pnp1 antibody
      • PNPH_HUMAN antibody
      • PRO1837 antibody
      • PUNP antibody
      • Purine nucleoside orthophosphate ribosyltransferase antibody
      • Purine nucleoside phosphorylase 5a antibody
      • Purine nucleoside phosphorylase antibody
      see all

    Anti-Nucleoside phosphorylase antibody [EPR5715] 画像

    • All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : JAR lysate
      Lane 3 : K562 lysate
      Lane 4 : Human placenta lysate
      Lane 5 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 32 kDa
      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    • ab109447 at 1/100 dilution staining Nucleoside phosphorylase in SH SY5Y cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) 使用論文

    ab109447 has not yet been referenced specifically in any publications.

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